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Key Documents

MAB2075Z

Sigma-Aldrich

Anti-Integrin β6 Antibody, clone R6G9, azide free

clone R6G9, Chemicon®, from mouse

Sinónimos:

MAB2075

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

R6G9, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2a

suitability

not suitable for Western blot
not suitable for activity/function inhibition

NCBI accession no.

NM_000888.3

UniProt accession no.

P18564

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ITGB6(3694)

Categorías relacionadas

Specificity

Monoclonal antibody MAB2075Z, raised by immunizing Balb/c mice with murine NIH 3T3 cells stably transfected with the human beta6 subunit, recognizes the human beta6 subunit by flow cytometry and is an excellent antibody for immunoprecipitation.

Immunogen

Murine NIH 3T3 cells stably transfected with the human (6 subunit

Application

Anti-Integrin β6 Antibody, clone R6G9, azide free is an antibody against Integrin β6 for use in FC & IP.
Flow cytometry

Immunoprecipitation: Effective; however because this antibody is an IgG2a, protein G or rabbit anti-mouse IgG is helpful for capture.

Immunohistochemistry/immunocytochemistry: The antibody recognizes alphaVbeta6 by immunohistochemistry and immunocytochemistry (acetone fix), but nuclear background staining limits usefulness in these techniques.


Then antibody is not function blocking and not effective for western blot.

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Alison Burman et al.
Journal of virology, 80(19), 9798-9810 (2006-09-16)
Foot-and-mouth disease virus (FMDV) can use a number of integrins as receptors to initiate infection. Attachment to the integrin is mediated by a highly conserved arginine-glycine-aspartic acid (RGD) tripeptide located on the GH loop of VP1. Other residues of this
A Weinacker et al.
The Journal of biological chemistry, 269(9), 6940-6948 (1994-03-04)
The integrin alpha v beta 6 has been shown to be a fibronectin-binding protein. To determine whether the cytoplasmic and transmembrane domains of alpha v beta 6 are necessary for binding to fibronectin, a truncated, secreted form of the integrin
Liudmila S Chesnokova et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(48), 20464-20469 (2009-11-19)
Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that is causally implicated in the development of lymphoid and epithelial tumors. Entry of virus requires fusion of virus envelopes and cell membranes. Fusion with B lymphocytes requires virus glycoprotein gB and
John J Grzesiak et al.
International journal of cancer, 129(12), 2905-2915 (2011-04-15)
To address the role of β(1) integrins in pancreatic cancer progression, we stably knocked down β(1) integrin subunit expression in human FG-RFP pancreatic cancer cells using lentiviral-based RNA interference. We then examined the effects of β(1) integrin

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