MAB131873
Anti-mCherry Antibody, clone 1C51
clone 1C51, from mouse
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
1C51, monoclonal
species reactivity (predicted by homology)
all
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
General description
mCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of Discosoma species. It is monomeric form that has been engineered with multiple cycles of mutation, directed modification and evolutionary selection. It exhibits excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry displays high photostability and resistant to photobleaching. It also displays improved brightness and extremely rapid maturation rate. Anti-mCherry, clone1C51 is an affinity purified antibody that recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref.: Schaner, NC et al. (2004). Nat. Biotechnol. 22(12):1567-72).
Specificity
Clone 1C51 detects mCherry protein in multiple species.
Immunogen
His-tagged full length recombinant mCherry.
Application
Anti-mCherry Antibody, clone IC51, Cat. No. MAB131873 detects mCherry protein and is tested for use in Immunocytochemistry and Western Blotting.
Western Blotting Analysis: A representative lot detected mCherry in Western Blotting applications (Krajcovic, M., et. al. (2013). Mol Biol Cell. 24(23):3736-45).
Western Blotting Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vs HEK293 untransfected cells (Courtesy of Dr Gerry Shaw).
Immunocytochemistry Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry (Courtesy of Dr Gerry Shaw).
Western Blotting Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vs HEK293 untransfected cells (Courtesy of Dr Gerry Shaw).
Immunocytochemistry Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry (Courtesy of Dr Gerry Shaw).
Quality
Evaluated by Western Blotting in HEK293 cells transfected with pFin-EF1-mCherry.
Western Blotting Analysis: 1 µg/mL of this antibody detected mCherry in 10 µg of lysate from HEK293 cells transfected with pFin-EF1-mCherry.
Western Blotting Analysis: 1 µg/mL of this antibody detected mCherry in 10 µg of lysate from HEK293 cells transfected with pFin-EF1-mCherry.
Target description
~32 kDa Observed; 26.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing PBS with 0.03% sodium azide with 50% glycerol.
Other Notes
Concentration: Please refer to lot specific datasheet.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Memoona Ramzan et al.
Human mutation, 42(10), 1321-1335 (2021-07-16)
Hereditary deafness is clinically and genetically heterogeneous. We investigated deafness segregating as a recessive trait in two families. Audiological examinations revealed an asymmetric mild to profound hearing loss with childhood or adolescent onset. Exome sequencing of probands identified a homozygous
Jae Jin Kim et al.
Molecular cell, 80(2), 327-344 (2020-09-24)
Stabilization of stalled replication forks is a prominent mechanism of PARP (Poly(ADP-ribose) Polymerase) inhibitor (PARPi) resistance in BRCA-deficient tumors. Epigenetic mechanisms of replication fork stability are emerging but remain poorly understood. Here, we report the histone acetyltransferase PCAF (p300/CBP-associated) as
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico