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Key Documents

CBL496F

Sigma-Aldrich

Anti-CD34 Class II Antibody, clone QBEND/10, FITC conjugated

clone QBEND/10, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

QBEND/10, monoclonal

species reactivity

monkey, human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD34(947)

Specificity

The antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. The antigen is expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. This antibody recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.

FUSION PARTNER: NSO myeloma cell line

Immunogen

Human endothelial vesicles

Application

Identification of haemopoietic progenitor cells

Studies into the control mechanisms of early haemopoiesis

Purification of haemopoietic cells from, for example, FACS

The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts

Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-CD34 Antibody (Class II), clone QBEND/10, FITC conjugated is validated for use in FC for the detection of CD34 Class II.

Physical form

The conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.

Storage and Stability

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Marlen Kolbe et al.
Tissue engineering. Part A, 17(17-18), 2199-2212 (2011-05-03)
Mesenchymal stem cells (MSC) from bone marrow and outgrowth endothelial cells (OEC) from peripheral blood are considered as attractive cell types for applications in regenerative medicine aiming to build up complex vascularized tissue-engineered constructs. MSC provide several advantages such as
L Fina et al.
Blood, 75(12), 2417-2426 (1990-06-15)
All seven of a set of CD34 monoclonal antibodies that recognize epitopes on an approximately 110 Kd glycoprotein on human hemopoietic progenitor cells also bind to vascular endothelium. Capillaries of most tissues are CD34 positive, as are umbilical artery and

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