ABN368

Anti-Spastin Antibody

serum, from rabbit

• Sinónimos: Spastin, Spastic paraplegia 4 protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, rat, mouse
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_055761
• UniProt accession no.: Q9UBP0
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SPAST(6683)

Descripción

General description

Spastin is a distinct microtubule-severing protein. Autosomal dominant mutations in the SPG4 locus corresponding to spastin are the most common cause of hereditary spastic paraplegia (HSP), a neurodegenerative disease that afflicts the adult corticospinal tracts.

Immunogen

Recombinant protein corresponding to human Spastin with 4 isoforms.

Application

Detect Spastin using this rabbit polyclonal antibody, Anti-Spastin Antibody validated for use in western blotting & ICC.

Western Blotting Analysis: A representative lot from an independent laboratory detected Spastin in myc-Spastin transfected HeLa and MRC5 cell lysates (Connell, et al. (2009). Traffic. 10:42-56).

Quality

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Spastin in 10 µg of L6 cell lysate.

Target description

~ 68, 64, 58, 55 kDa observed. Uniprot describes 4 isoforms produced by alternative splicing at ~68 kDa, ~64 kDa, ~58 kDa, and ~55 kDa Spastin has shown in some western blots that four isoforms [full length (68 kD), exon 4-deleted full length (64 kD), short form (58-60 kD) and exon 4-deleted short form (55 kD)] are expressed in mammalian cells. Ths pAb detects bands corresponding to the size of these four isoforms in HeLa, MRC5 human lung fibroblast and NSC34 mouse lower motor neuron neuroblastoma fusion cell lines (Connell, et al. (2009). Traffic. 10:42-56).

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1