ABE1391
Anti-Brd4 Antibody
serum, from rabbit
Sinónimos:
Bromodomain-containing protein 4, Mitotic chromosome-associated protein, MCAP, Brd4
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
species reactivity (predicted by homology)
mouse
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Brd4(57261)
General description
Bromodomain-containing protein 4 (Brd4), or Mitotic chromosome-associated protein (MCAP), is a chromatin reader protein and kinase that recognizes and binds acetylated histones and plays a key role in the transmission of epigenetic memory across cell divisions and transcription regulation. Brd4 remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for post mitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, Brd4 plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Brd4 is widely expressed and localized to the nucleus and acetylated chromatin. Brd4 also plays a large role in many cancers, and through its regulation of transcription, Brd4 is important in multiple cellular processes including DNA damage responses, aging, and development.
Specificity
Recognizes human Brd4
Immunogen
Epitope: Region between amino acids 150 to 290 of human Brd4
Recombinant protein corresponding to Mouse Brd4 short form.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Histones
Chromatin Biology
Histones
This Anti-Brd4 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Immunocytochemistry for the detection of Brd4.
Western Blotting Analysis: A representative lot of this antibody was reported to detect Brd4 protein in a HeLa cell lysate (Alsarraj J., et al. (2013). Also see Anup, D., et al. (2003) and Mochizuki, K., et al. (2008) for additional data using mouse embyronic fibroblasts and NIH3T3 cells.
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP using chromatin from NIH3T3 cells. See Mochizuki, K., et al. (2008) and Anup, D., et al. (2009).
Immunocytochemistry: A representative lot of this antibody was reported to work in ICC. See Anup, D., et al. (2000) and Anup, D., et al. (2009).
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP using chromatin from NIH3T3 cells. See Mochizuki, K., et al. (2008) and Anup, D., et al. (2009).
Immunocytochemistry: A representative lot of this antibody was reported to work in ICC. See Anup, D., et al. (2000) and Anup, D., et al. (2009).
Quality
Evaluated by Western Blotting in HEK293 cells.
Western Blotting Analysis: 1:1,000 dilution of this antibody detected Brd4 in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: 1:1,000 dilution of this antibody detected Brd4 in 10 µg of HEK293 cell lysate.
Target description
~ 100 kDa observed
This antibody primarily detects 100 kd short form of Brd4 and to lesser extent larger 180 kD form (see Alsarraj J., et al. (2013 for additional details on performance of this antibody).
This antibody primarily detects 100 kd short form of Brd4 and to lesser extent larger 180 kD form (see Alsarraj J., et al. (2013 for additional details on performance of this antibody).
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Referencia del producto
Descripción
Precios
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
A Dey et al.
Molecular and cellular biology, 20(17), 6537-6549 (2000-08-11)
We describe a novel nuclear factor called mitotic chromosome-associated protein (MCAP), which belongs to the poorly understood BET subgroup of the bromodomain superfamily. Expression of the 200-kDa MCAP was linked to cell division, as it was induced by growth stimulation
Anup Dey et al.
Molecular biology of the cell, 20(23), 4899-4909 (2009-10-09)
On entry into mitosis, many transcription factors dissociate from chromatin, resulting in global transcriptional shutdown. During mitosis, some genes are marked to ensure the inheritance of their expression in the next generation of cells. The nature of mitotic gene marking
C A French et al.
The American journal of pathology, 159(6), 1987-1992 (2001-12-26)
Translocation t(15;19)(q13;p13.1) defines a lethal midline carcinoma arising adjacent to respiratory tract in young people. To characterize molecular alterations responsible for the distinctly aggressive biological behavior of this cancer, we mapped the chromosome 15 and 19 translocation breakpoints by fluorescence
Kazuki Mochizuki et al.
The Journal of biological chemistry, 283(14), 9040-9048 (2008-01-29)
Brd4 is a bromodomain protein that binds to acetylated chromatin. It regulates cell growth, although the underlying mechanism has remained elusive. Brd4 has also been shown to control transcription of viral genes, whereas its role in transcription of cellular genes
Talha Ijaz et al.
Cell death & disease, 8(2), e2606-e2606 (2017-02-10)
Transdifferentiation of quiescent dermal fibroblasts to secretory myofibroblasts has a central role in wound healing and pathological scar formation. This myofibroblast transdifferentiation process involves TGFβ-induced de novo synthesis of alpha smooth muscle cell actin (αSMA)+ fibers that enhance contractility as
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