ABC124
Anti-phospho-ULK1 (Ser555) Antibody
from rabbit, purified by affinity chromatography
Sinónimos:
Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)
inhibition assay: suitable (peptide)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer555)
Gene Information
human ... ULK1(8408)
General description
ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.
Specificity
This antibody recognizes ULK1 phosphorylated at Ser555.
Immunogen
Epitope: Phosphorylated Ser555
KLH-conjugated linear peptide corresponding to human ULK1 phosphorylated at Ser555.
Application
Peptide Inhibition Analysis: A representative lot of this antibody blocked ULK1 in PIA involving peptide 3123-C vs. peptide 3123 on serum starved MCF7 cell lysates.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Kinases & Phosphatases
Kinases & Phosphatases
This Anti-phospho-ULK1 (Ser555) Antibody is validated for use in Western Blotting, Peptide Inhibition Assay for the detection of phospho-ULK1 (Ser555).
Quality
Evaluated by Western Blot in non-serum starved and serum starved MCF7 cell lysates.
Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-ULK1 (Ser555) in 10 µg of non-serum starved and serum starved MCF7 cell lysates.
Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-ULK1 (Ser555) in 10 µg of non-serum starved and serum starved MCF7 cell lysates.
Target description
~120 kDa observed. Uniprot describes a molecular weight at 112 kDa This protein has been observed at greater than ~125 kDa due to multiple phosphorylation-dephosphorylation processes. (Ganley, I. G., et al. (2009). J Biol Chem. 284(18):12297-12305.)
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Non-serum starved and serum starved MCF7 cell lysates
Non-serum starved and serum starved MCF7 cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Optional
Referencia del producto
Descripción
Precios
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
PHY906(KD018), an adjuvant based on a 1800-year-old Chinese medicine, enhanced the anti-tumor activity of Sorafenib by changing the tumor microenvironment.
Lam, W; Jiang, Z; Guan, F; Huang, X; Hu, R; Wang, J; Bussom, S; Liu, SH; Zhao, H; Yen, Y; Cheng, YC
Scientific Reports null
Aleksandra V Borodkina et al.
Cell cycle (Georgetown, Tex.), 15(1), 117-127 (2015-12-05)
Previously we demonstrated that endometrium-derived human mesenchymal stem cells (hMESCs) via activation of the ATM/p53/p21/Rb pathway enter the premature senescence in response to oxidative stress. Down regulation effects of the key components of this signaling pathway, particularly ATM and p53
Aleksandra V Borodkina et al.
Aging, 8(12), 3400-3418 (2016-12-13)
Intracellular calcium ([Ca2+]i) has been reported to play an important role in autophagy, apoptosis and necrosis, however, a little is known about its impact in senescence. Here we investigated [Ca2+]i contribution to oxidative stress-induced senescence of human endometrium-derived stem cells
Prasun Guha et al.
Cell reports, 26(10), 2692-2703 (2019-03-07)
Autophagy plays a broad role in health and disease. Here, we show that inositol polyphosphate multikinase (IPMK) is a prominent physiological determinant of autophagy and is critical for liver inflammation and regeneration. Deletion of IPMK diminishes autophagy in cell lines
Elena Y Kochetkova et al.
Aging, 9(11), 2352-2375 (2017-11-16)
The Ras-Raf-MEK-ERK pathway plays a central role in tumorigenesis and is a target for anticancer therapy. The successful strategy based on the activation of cell death in Ras-expressing cells is associated with the suppression of kinases involved in Ras pathway.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico