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71772

Millipore

ProteoExtract® Transmembrane Protein Extraction Kit

Sinónimos:

Membrane Protein Isolation Kit

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.77

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

2-8°C

General description

The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).

Application

ProteoExtract® Transmembrane Protein Extraction Kit has been used: for extraction of protein from the apex segment of the colon in western blot analysis of Na+/H+ exchanger (NHE3) for extracting plasma membrane proteins

Features and Benefits

  • No sonication, intensive vortexing, lengthy ultracentrifugation, or high-temperature incubation is needed.
  • Reagents are sufficient to process a total of 40 samples.

Warning

Toxicity: Toxic (F)

Other Notes

Nakamura, E., et al. 2008. Cancer Sci.99, 1390.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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