524736

Phosphodiesterase 4B2, His•Tag^®, Human, Recombinant, S. frugiperda

• Sinónimos: PDE4B2, cAMP-Specific Phosphodiesterase 4B2
• UNSPSC Code: 12352202
• NACRES: NA.54

Propiedades

• recombinant: expressed in baculovirus infected Spodoptera frugiperda cells
• Quality Level: 100
• form: liquid
• specific activity: ≥0.25 U/μL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Descripción

General description

Full-length, recombinant, human phosphodiesterase 4B2 (accession no. L20971) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda using a baculovirus expression system. Plays a significant role in the regulation of inflammatory cell activation. PDE4B2 is a predominant isoform in neutrophils and monocytes. Supplied as a partially purified, catalytically active preparation.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.

Physical form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Smith, P.G., et al. 2005 Blood105, 308.
Baroja, M.L., et al. 1999. J. Immunol.162, 2016.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable