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Supelco

SeQuant ZIC®-pHILIC (5 µm) HPLC Columns

L x I.D. 20 mm x 2.1 mm guard Column Kit (3 pc) with coupler (1 pc)

Sinónimos:

Hydrophilic Interaction Liquid Chromatography Guard Column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

SeQuant ZIC®-pHILIC Guard Kit 20 x 2.1 mm, PEEK-lined stainless steel column, pkg of 3 column

material

PEEK-lined stainless steel column

Quality Level

agency

suitable for USP L122

product line

SeQuant

feature

endcapped: no

packaging

pkg of 3 column

parameter

200 bar pressure
50 °C max. temp.

technique(s)

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

2 cm × 2.1 mm

matrix

polymeric particle platform

matrix active group

Zwitterion bonding phase, ZIC-pHILIC

particle size

5 μm

pore size

100 Å pore size

operating pH

2-10

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

General description

The Guard column kit contains 3 SeQuant ZIC®-pHILIC guard columns including the fitting to connect to the analytical column.SeQuant ZIC®-pHILIC stationary phase has the same highly polar, bonded, permanent zwitterion, functional group as the silica based ZIC®-HILIC Column. The user can therefore expect the same selectivity, however, with a trade-off in flow-rate range, and separation efficiency, common with polymeric materials. The more durable support allows for use in an extended pH range, which can be beneficial for certain applications.SeQuant ZIC®-pHILIC HPLC columns are the preferred choice when extreme eluent conditions are needed to overcome challenging separations of polar hydrophilic compounds. Offering excellent pH stability from pH 2 to 10, these polymer-based columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. This results in straightforward and reproducible separations with wide-spectrum pH tuning for optimal separation selectivity and detection sensitivity. Furthermore, SeQuant ZIC®-pHILIC columns minimize background noise in evaporative detection techniques with high sensitivity to ions, such as charged aerosol detectors (CAD). SeQuant ZIC®-pHILIC columns are available with 5 μm polymer particles in various formats of analytical dimensions. Alternative choices for the separation of polar hydrophilic compounds include silica-based SeQuant ZIC®-HILIC columns with identical functional groups, and SeQuant ZIC®-cHILIC columns with complementary selectivity.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

performance test cytosine
Extent of testing: conforms

Legal Information

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

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