126850

α₁-PDX, Human, Recombinant, E. coli

Recombinant protein derived from the bioengineered human α1-antitrypsin gene fused to a His•Tag sequence and a FLAG-tag.

• Sinónimos: α₁-Antitrypsin Portland
• UNSPSC Code: 12352200
• NACRES: NA.77

Propiedades

• Quality Level: 100
• assay: ≥80% (SDS-PAGE)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• solubility: water: 2.5 mg/mL
• shipped in: wet ice
• storage temp.: 2-8°C

Descripción

General description

Recombinant protein derived from the bioengineered human α1-antitrypsin gene fused to a His·Tag and a FLAG·Tag. Contains a minimal furin consensus sequence, RXXR, that effectively blocks the furin-dependent processing of protein precursors (K_i = 600 pM). α1-PDX can be used in extracellular applications to block the processing of furin substrates. α1-PDX has been shown to inhibit the processing of biologically significant proteins including viral envelope glycoproteins, bacterial toxins, receptors, and growth factors.

Recombinant protein derived from the bioengineered human α₁-antitrypsin gene fused to a His•Tag sequence and a FLAG-tag. Contains a minimal furin consensus sequence, RXXR, that effectively blocks the furin-dependent processing of protein precursors (K_i = 600 pM). Extracellular application of α₁-PDX can be used to inhibit the processing of biologically significant proteins including viral envelope glycoproteins, bacterial toxins, receptors, and growth factors. Useful for in vivo and in vitro furin inhibition assays.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Furin-dependent processing of protein precursors

Product does not compete with ATP.

Reversible: no

Target K_i: 600 pM against furin-dependent processing of protein precursors

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from PBS.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Bassi, D.E., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10326.
Jean, F., et al. 2000. Proc. Natl. Acad. Sci. USA97, 2864.
Jean, F., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7293.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable