126621
Bovine Serum Albumin
Cohn Fraction, 30% Aqueous Solution
Sinónimos:
Albumin, Bovine Serum, Cohn Fraction, 30% Aqueous Solution
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About This Item
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product name
Albumin, Bovine Serum, Cohn Fraction, 30% Aqueous Solution,
assay
≥95% (agarose gel electrophoresis)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
MALDI-TOF: suitable
shipped in
ambient
storage temp.
2-8°C
General description
Albumin, Bovine Serum, (BSA) Cohn Fraction is purified by cold ethanol precipitation (Cohn extraction) and by heat treatment to remove lipids and fatty acids. It is a single carbohydrate-free polypeptide and the most abundant (comprising ~60%) of the plasma proteins. BSA regulates the maintenance of osmotic pressure.
BSA belongs to the serum albumin family, comprising three domains with two sub-domains under each. BSA is an α-helical, globular, and non-glycosylated protein with 17-disulfide bonds.
Biochem/physiol Actions
Bovine serum albumin (BSA) transports sparingly soluble physiological substances, especially long-chain fatty acids, bilirubin, drugs, hormones, and fatty acids. It acts as a blocking agent in enzyme-linked immunosorbent assay (ELISA). BSA is a vital component of the cell culture media and boosts embryonic stem cells (hESC) differentiation.
Warning
Toxicity: Standard Handling (A)
Physical form
30% solution in 0.8% NaCl, 0.1% NaN₃, pH 7.2, sterile-filtered.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter
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