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1.00485

Sigma-Aldrich

Masson-Goldner staining kit

for the visualization of connective tissue with trichromic staining

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About This Item

UNSPSC Code:
41116124
NACRES:
NA.41

Quality Level

IVD

for in vitro diagnostic use

dilution

(for the visualization of connective tissue with trichromic staining)

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

The Masson-Goldner staining kit - for the visualization of connective tissue with trichromic staining, is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. Using a combination of three different staining solutions, muscle fibers, collagenous fibers, fibrin and erythrocytes can be selectively visualized.
The original methods were primarily used to differentiate collagenous and muscle fibers. The stains used have different molecular sizes and enable the individual tissues to be stained differentially.
The Masson-Goldner staining technique can be carried out using formalin fixed material. Subsequent to staining the nucleus with Weigert′s iron hematoxylin, components such as muscle, cytoplasm and erythrocytes are stained with azophloxin and orange G solution. Connective tissue is then counter stained using light green SF solution.
The package is sufficient for 400 - 500 applications. The product is registered as IVD and CE product and can be used in diagnostics and laboratory accreditation. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy (Tissue section): passes test
Nuclei: dark brown to black
Cytoplasm: brick red
musculature (muscles): bright red
Connective tissue: green
acid mucosubstances: green
Erythrocytes: bright orange

pictograms

Corrosion

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 2


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