07-1378

Anti-QIK (SIK2) Antibody

Upstate^®, from rabbit

• Sinónimos: Qin-induced kinase, SNF1-like kinase 2, Salt-inducible protein kinase 2, salt-inducible kinase 2, salt-inducible serine/threonine kinase 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, canine, mouse, chicken, bovine, human
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• NCBI accession no.: NP_056006
• UniProt accession no.: Q9H0K1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SIK2(23235)

Descripción

General description

QIK (Qin-induced Kinase, SNF1LK2, SIK2, Salt-induced protein Kinase 2) is a Ser/Thr kinase that belongs to the AMPK/SNF1 kinase family. QIK is phosphorylated and activated by the tumor suppressor LKB1 when in complex with the pseudokinase STRAD and the scaffolding protein MO25. QIK has been found in adipocytes where it is known to phosphorylate a few substrates that include insulin receptor substrate 1 (IRS1) on Ser794 and TORC2 (also known as CRTC2 and not to be confused with the mTOR complex) on Ser171. The phosphorylation of TORC2 results in its translocation to the nucleus where it binds to CREB.

Specificity

Close homology with mouse, rat, chicken, dog, and cow.

Detects QIK

Immunogen

KLH-conjugated synthetic peptide corresponding to the C-term of human QIK.

Application

Anti-QIK (SIK2) Antibody is an antibody against QIK (SIK2) for use in WB.

Research Category
Signaling

Quality

Western Blot Analysis:
This lot detected QIK at 1:1,000-1:2,000 dilution in HeLa lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).

Target description

104 kDa

Physical form

Format: Purified

Protein A purified

Protein A purified rabbit serum in 0.1M Tris-Glycine (pH7.4) 15mM NaCl, and 0.05% NaN3 with 30% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa Cell Lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1