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Merck
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Key Documents

05-983

Sigma-Aldrich

Anti-PKC Antibody (α, β, γ), clone M110

clone M110, Upstate®, from mouse

Sinónimos:

PRKCA, PRKCB, PRKCG, protein kinase C, alpha, beta, gamma

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

M110, monoclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NP_002728
NP_002730

UniProt accession no.

P05129
P05771
P17252

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PRKCA(5578)

Specificity

Recognizes PKC α, β, γ. Does not cross react with other PKC isoforms.

Immunogen

Recombinant protein of human PKCγ amino acids 499-697.

Application

Anti-PKC (α Antibody, β, γ), clone M110 detects level of PKC (α & has been published & validated for use in ELISA, IP, WB & IC.

Quality

Routinely evaluated by immunoblot.

Target description

80-82 kDa

Physical form

100 μL of protein A purified mouse monoclonal IgG1 in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
Format: Purified

Storage and Stability

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Descripción
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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Thomas T Su et al.
Nature immunology, 3(8), 780-786 (2002-07-16)
NF-kappa B signaling is required for the maintenance of normal B lymphocytes, whereas dysregulated NF-kappa B activation contributes to B cell lymphomas. The events that regulate NF-kappa B signaling in B lymphocytes are poorly defined. Here, we demonstrate that PKC-beta
Amos M Sakwe et al.
The Journal of biological chemistry, 280(6), 4436-4441 (2004-12-02)
This study examined the mechanism of Ca2+ entry and the role of protein kinase C (PKC) in Ca2+ signaling induced by activation of the calcium sensing receptor (CaR) in HEK293 cells stably expressing the CaR. We demonstrate that influx of
Michael Rosenberg et al.
Molecular biology of the cell, 17(3), 1364-1374 (2006-01-06)
Nonmuscle myosin II is an important component of the cytoskeleton, playing a major role in cell motility and chemotaxis. We have previously demonstrated that, on stimulation with epidermal growth factor (EGF), nonmuscle myosin heavy chain II-B (NMHC-IIB) undergoes a transient
Lilian Kisiswa et al.
Open biology, 7(1) (2017-01-20)
Tumour necrosis factor receptor 1 (TNFR1)-activated TNFα reverse signalling, in which membrane-integrated TNFα functions as a receptor for TNFR1, enhances axon growth from developing sympathetic neurons and plays a crucial role in establishing sympathetic innervation. Here, we have investigated the
Evolution of the eye transcriptome under constant darkness in Sinocyclocheilus cavefish.
Meng, F; Braasch, I; Phillips, JB; Lin, X; Titus, T; Zhang, C; Postlethwait, JH
Molecular Biology and Evolution null
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