810603P
Avanti
16:0-10 Doxyl PC
Avanti Research™ - A Croda Brand 810603P, powder
Sinónimos:
1-palmitoyl-2-stearoyl-(10-doxyl)-sn-glycero-3-phosphocholine
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About This Item
Fórmula empírica (notación de Hill):
C46H90N2O10P
Número de CAS:
Peso molecular:
862.19
MDL number:
UNSPSC Code:
41141825
NACRES:
NA.25
Productos recomendados
assay
>99% (TLC)
form
powder
packaging
pkg of 1 × 1 mg (810603P-1mg)
manufacturer/tradename
Avanti Research™ - A Croda Brand 810603P
lipid type
ESR probes
phospholipids
shipped in
dry ice
storage temp.
−20°C
General description
Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.
Phosphocholine is considered as a precursor molecule. It is formed during the breakdown of phosphatidylcholine metabolism. 10-Doxyl is a spin label probe.
Application
16:0-10 Doxyl PC may be used:
- in vesicles for quenching PT-(1–46)F4W fluorescence
- as vesicle component for tryptophan based fluorescence emission studies
- to probe the oxygen and Ni[II] ethylenediaminediacetic acid (NiEdda) accessibilities inside liposomes
Biochem/physiol Actions
Phosphatidylcholine (PC) functions as a surfactant within the mucus to form a hydrophobic surface to inhibit bacterial penetrance.
Packaging
5 mL Clear Glass Sealed Ampule (810603P-1mg)
Preparation Note
Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
Storage Class
11 - Combustible Solids
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Integrative Medicine - E-Book (2017)
Alexander Kyrychenko et al.
The journal of physical chemistry. B, 117(19), 5875-5885 (2013-04-26)
Spin-labeled lipids are commonly used as fluorescence quenchers in studies of membrane penetration of dye-labeled proteins and peptides using depth-dependent quenching. Accurate calculations of depth of the fluorophore rely on the use of several spin labels placed in the membrane
Delivery of nitroxide spin label to cultured cells by liposomes
Chan HC
Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, 8, 160-170 (1988)
Phosphatidylserine dynamics in cellular membranes.
Kay, J.G
Molecular Biology of the Cell, 23, 2198-2212 (2012)
Kinetics of antibody-dependent activation of the first component of complement on lipid bilayer membranes
Parce JW
Biochemical and Biophysical Research Communications, 93, 235-242 (1980)
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