G006149

UHPLC/MS Analysis of Various Drug Substances on Titan^™ C18

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: Titan C18, 5 cm x 2.1 mm I.D., 1.9 μm particles (577122-U); mobile phase: [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 90:10 (v:v) acetonitrile:water; gradient: 30 to 70% B in 3.0 min; flow rate: 0.3 mL/min; column temp.: 35 °C; detector: MS/MS; injection: 3 μL; sample: 50 ng/mL in methanol
• suitability: application for HPLC
• application(s): forensics and toxicology; pharmaceutical (small molecule)

Description

Analysis Note

A Titan C18 column provided rapid, baseline resolution of this set of drug substances. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Analyte

Metoprolol tartrate solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant^®
Propranolol hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant^®
Carbamazepine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Diazepam solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®