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G006142

HPLC Analysis of Proteins and Peptides on Proteomix® WCX-NP1.7 Demonstrating Lot-to-Lot Reproducibility

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Proteomix WCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777180)
mobile phase: (A) 20 mM sodium phosphate, pH 6.5; (B) A + 1.0 M NaCl
gradient: 0 to 100% B in 25 min, 15 min prewash
flow rate: 0.5 mL/min
column temp.: ambient
detector: UV, 214 nm
injection: 5 μL
sample: 1 mg/mL

suitability

application for HPLC

application(s)

life science and biopharma
pharmaceutical (small molecule)

Legal Information

Proteomix is a registered trademark of Sepax Technologies

Analyte

Description
    Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
    Cytochrome c from chicken heart, ≥95% based on Mol. Wt. 12,222 basis
    Lysozyme from chicken egg white, powder or granules, ≥90 %, ≥39,000 units/mg protein

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