G005940
HPLC Analysis of Monoclonal Antibodies (mAb) with Charge Variance on Antibodix® WCX-NP10
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 μm particles (Z777272)
mobile phase: (A) 10 mM sodium phosphate buffer, pH 7.5; (B) A + 100 mM NaCl
gradient: 15 to 55% B in 30 min
flow rate: 0.8 mL/min
pressure: 840 psi (58 bar)
column temp.: ambient
detector: UV, 214 nm
injection: 5 μL
sample: monoclonal antibody
suitability
application for HPLC
application(s)
life science and biopharma
pharmaceutical (small molecule)
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