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ZRB1317

Sigma-Aldrich

Anti-Synaptophysin Antibody, clone 1G7, ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):

Major synaptic vesicle protein p38

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

rabbit (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1G7, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 33.31 kDa
observed mol wt ~35 kDa

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ZRB1365ZRB1450ZRB1309
Gene Information

human ... SYP(6855)

Gene Information

human ... SYP(6855)

Gene Information

human ... PIAS1(8554)

Gene Information

human ... DMD(1756)

clone

1G7, monoclonal, recombinant monoclonal

clone

2B21, monoclonal, recombinant monoclonal

clone

1O17, monoclonal, recombinant monoclonal

clone

1G7, monoclonal, recombinant monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

species reactivity

human, mouse, rat

species reactivity

rat, human

species reactivity

human, mouse

species reactivity

mouse, human

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1G7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Synaptophysin. It targets an epitope within 86 amino acids from the C-terminal, cytoplasmic domain.

Immunogen

GST/His-tagged Recombinant Protein corresponding to 86 amino acids from the C-terminal, cytoplasmic region of Rat Synaptophysin.

Application

Anti-Synaptophysin, clone 1G7 ZooMAb, Cat. No. ZRB1317, is a recombinant rabbit monoclonal antibody that detects Synaptophysin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Synaptophysin in rat brain, human brain, and PC12 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Synaptophysin in rat brain and human brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Synaptophysin in PC12 and E18 rat cortical cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Synaptophysin (UniProt: P07825; also known as Major synaptic vesicle protein p38) is encoded by the Syp gene (Gene ID: 24804) in rat. Synaptophysin belongs to the synaptophysin/synaptobrevin family of proteins. It is one of the major glycoproteins present in presynaptic vesicles and in various neuroendocrine cells of both neuronal and epithelial phenotype. Its structure consists of a hexameric protein of four transmembrane spans with a short cytoplasmic amino and a long carboxy-terminal tail. Both amino- and carboxy-terminals are exposed on the cytoplasmic surface of the synaptic vesicle membrane. C-terminal domain of Synaptophysin is selectively required for the endocytosis during, but not after, cessation of sustained synaptic transmission. Synaptophysin is involved in organizing membrane components may regulate endocytosis to ensure vesicle availability following sustained neuronal activity. It is also involved in the regulation of short-term and long-term synaptic plasticity. Syp/neurons display functional deficiencies and slower recovery of the recycling synaptic vesicle pool. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kwon, SE and Chapman, ER (2011). Neuron. 70(5): 847-854).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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