ZMS1164
Anti-CDKL5/STK9 Antibody, clone 8F3.1 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Cyclin-dependent kinase-like 5, EC:2.7.11.22, STK9, Serine/threonine-protein kinase 9
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
recombinant monoclonal
description
clone 8F3.1
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 107.5 kDa
observed mol wt ~120 kDa
purified by
using protein G
species reactivity
mouse, human
species reactivity (predicted by homology)
rat
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
western blot: suitable
isotype
IgG1κ
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... CDKL5(6792)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 8F3.1 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Cyclin-dependent kinase-like 5 (CDKL5). It targets an epitope within the internal region.
Immunogen
GST-tagged recombinant fragment from the internal region of human Cyclin-dependent kinase-like 5 (CDKL5).
Application
Quality Control Testing
Evaluated by Western Blotting in Mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CDKL5/STK9 in Mouse brain tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected c-Myc/DDK-tagged, full-length, Human recombinant CDKL5.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CDKL5/STK9 in Mouse brain tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected c-Myc/DDK-tagged, full-length, Human recombinant CDKL5.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Cyclin-dependent kinase-like 5 (EC 2.7.11.22; UniProt O76039; also known as Serine/threonine-protein kinase 9) is encoded by the CDKL5 (also known as EIEE2, STK9) gene (Gene ID: 6792) in human. CDKL5, a proline-directed serine/threonine kinase that plays a role in regulating axon outgrowth, dendritic morphogenesis, and synapse formation in early postnatal life, and in maintaining synaptic function in the adult brain. It is detected in the nucleus and dendrites of neurons. Its expression appears to be low in the embryonic cortex and is strongly induced at perinatal and postnatal stages in maturing neurons. It is reported to shuttle between the cytoplasm and nucleus through a CRM1-mediated nuclear export mechanism. Its protein kinase domain is localized in amino acids 13-297 and the serine-threonine protein kinase active site resides in amino acids 127-144. It has large C-terminal extension of about 700 amino acids that has several functions. This region serves as a negative regulator of the catalytic activity and is also essential for its nuclear localization. Mutations in CDKL5 gene have been linked to developmental and epileptic encephalopathy 2 (DEE2), a severe form of epilepsy characterized by seizures or spasms beginning in infancy. Two isoforms of CDKL5 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Leonard, H., et al. (2022). Lancet Neurology. 21(6); 563-576; Bahi-Buisson, N., and Bienvenu, T. (2012). Mol. Syndromol. 2(3-5); 137-152).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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