V9131
Anti-Vinculin Antibody
mouse monoclonal, hVIN-1
Synonym(s):
Anti Vinculin, Vinculin Antibody, Vinculin Antibody - Monoclonal Anti-Vinculin antibody produced in mouse
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Product Name
Monoclonal Anti-Vinculin antibody produced in mouse, clone hVIN-1, ascites fluid
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
hVIN-1, monoclonal
mol wt
antigen 116 kDa
contains
15 mM sodium azide
species reactivity
frog, chicken, mouse, canine, human, bovine, rat, turkey
General description
Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions and the membrane-associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.
Specificity
Monoclonal Anti-Vinculin specifically stains vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells using indirect immunofluorescent labeling. The antibody reacts with the 116 kDa vinculin band in immunoblotting. The product reacts with vinculin of many species. Good reactivity is obtained with human, bovine, chicken, dog, rat, mouse, turkey, and Xenopus. The antibody shows cross reactivity with smooth muscle metavinculin.
Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.
Immunogen
purified human vinculin from uterus.
Application
Monoclonal Anti-Vinculin antibody produced in mouse has been used in:
- western blotting
- immunofluorescence staining
- immunohistochemistry
Biochem/physiol Actions
Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the β-subunit of integrin binds to talin. Talin binds to vinculin that interacts with α-actinin and possibly with itself. Since α-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Melusine Bleu et al.
Nature communications, 10(1), 3739-3739 (2019-08-23)
Transcription factor networks shape the gene expression programs responsible for normal cell identity and pathogenic state. Using Core Regulatory Circuitry analysis (CRC), we identify PAX8 as a candidate oncogene in Renal Cell Carcinoma (RCC) cells. Validation of large-scale functional genomic
Dystrophin-glycoprotein complex and vinculin-talin-integrin system in human adult cardiac muscle
Anastasi G, et al.
International Journal of Molecular Medicine, 23(2), 149-159 (2009)
Reinforcement versus fluidization in cytoskeletal mechanoresponsiveness
Krishnan R, et al.
PLoS ONE, 4(5), e5486-e5486 (2009)
Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure
Minamishima YA, et al.
Blood, 111(6), 3236-3244 (2008)
Vinculin and talin: focus on the myocardium
Zemljic-Harpf A, et al.
Journal of Investigative Medicine, 57(8), 849-855 (2009)
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