SAB4200048

Anti-E1B-AP5 antibody, Rat monoclonal

clone EAP4A11, purified from hybridoma cell culture

• Synonym(s): Anti-Adenovirus E1B 55-KD protein-associated protein 5, Anti-Heterogeneous nuclear ribonucleoprotein U-like 1, Anti-hnRNPUL1, Anti-hnRPUL1, Monoclonal Anti-E1B-AP5 antibody produced in rat
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: EAP4A11, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~110 kDa
• species reactivity: bovine, canine, human, rat, monkey, mouse
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.05-0.1 μg/mL using HT-29 cell extracts
• isotype: IgG2b
• UniProt accession no.: Q9BUJ2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HNRNPUL1(11100)

Description

General description

Monoclonal Anti-E1B-AP5 (rat IgG2b isotype) is derived from the hybridoma EAP4A11 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with full length human E1B-AP5 fusion protein. E1B-55K-associated protein 5 (E1B-AP5), also known as heterogeneous nuclear ribonucleoprotein U-like 1(hnRNPUL1), is a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family.

The E1B-AP5 gene is located on the human chromosome at 19q13.2.

Specificity

Monoclonal Anti-E1B-AP5 recognizes human, monkey, bovine, dog, mouse and rat E1B-AP5.

Immunogen

full length human E1B-AP5 fusion protein.

Application

Monoclonal Anti-E1B-AP5 antibody produced in rat may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry

Biochem/physiol Actions

E1B-55K-associated protein 5 (E1B-AP5) plays a vital role in ATR signaling. In addition, it may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B55kDa in adenovirus-infected cells. Interestingly, this protein was found to be associated with bromodomain-containing protein, BRD7 in a complex that links chromatin events with mRNA processing at the level of transcriptional regulation. It also activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable