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N6019

Sigma-Aldrich

Neutralizing Buffer

for Northern and Southern blotting, powder

Synonym(s):

Neutralizing Buffer for Blotting Techniques

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25
Pricing and availability is not currently available.

grade

for molecular biology

Quality Level

200

form

powder

technique(s)

Southern blotting: suitable

foreign activity

DNase, RNase, none detected

storage temp.

room temp

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This Item
S8140S6639G2526
Neutralizing Buffer for Northern and Southern blotting, powder

N6019

Neutralizing Buffer

SSPE Buffer for Northern and Southern blotting, powder blend

S8140

SSPE Buffer

SSC Buffer 20× Concentrate for Northern and Southern blotting, solution

S6639

SSC Buffer 20× Concentrate

Gel Loading Buffer for NA electrophoresis

G2526

Gel Loading Buffer

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder blend

form

solution

form

liquid

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

foreign activity

DNase, RNase, none detected

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

RNAse, none detected

General description

Sigma′s neutralization buffer is suitable for Southern blotting procedures [1] to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.

Application

Suitable for neutralizing the gel before transfer to membranes during Southern procedures.

Components

The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLBP9917V
    SLBW3389
    SLBT7455
    SLBP0163V
    SLBN5770V

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    A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
    Cai D, et al.
    Methods in Molecular Biology, 151-161 (2013)
    Sambrook, J., and Russell, D. W.
    Molecular Cloning: A Laboratory Manual, 6-6 (2001)
    Ausubel, F.M., et al.
    Short Protocols in Molecular Biology (1995)
    Sambrook, J., et al.
    Molecular Cloning: A Laboratory Manual, 9-9 (1989)

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