MAK525
Acid Phosphatase Fluorometric Assay Kit
sufficient for 100 fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
Quality Level
detection method
fluorometric
relevant disease(s)
genitourinary disorders; cancer; gastrointestinal diseases
shipped in
wet ice
storage temp.
−20°C
General description
Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.
Features and Benefits
- Linear detection range: 0.008 to 10 U/L .
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for acid phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples).
Principle
The Acid Phosphatase Fluorometric Assay Kit is based on the cleavage of a synthetic substrate. The product methylumbelliferone (MUB) becomes intensely fluorescent after addition of the stop reagent. The increase in fluorescence at λEx = 360 nm/λEm = 450 nm after addition of the stop reagent is directly proportional to the enzyme activity.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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