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M6018

Sigma-Aldrich

4-Methylumbelliferyl β-D-cellobioside

glucanase substrate

Synonym(s):

4-Methylumbelliferyl beta-D-cellobioside

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About This Item

Empirical Formula (Hill Notation):
C22H28O13
CAS Number:
72626-61-0
Molecular Weight:
500.45
EC Number:
276-744-7
MDL number:
MFCD00063279
UNSPSC Code:
12352204
PubChem Substance ID:
24897067
NACRES:
NA.32

Quality Level

200

Assay

≥98.00% (TLC)

form

powder

solubility

water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15-,16+,17-,18-,19-,20-,21-,22+/m1/s1

InChI key

PRTGXBPFDYMIJH-MKQZUAMYSA-N

Application

4-Methylumbelliferyl β-D-cellobioside has been used as a substrate:
  • to assay cellulase from A. niger
  • to determine the extracellular enzyme activity for 1,4-β-cellobiohydrolase
  • to assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reesei class II hydrophobins (HFBI)

Biochem/physiol Actions

4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G P Xue et al.
Journal of general microbiology, 138(11), 2397-2403 (1992-11-01)
A plant polysaccharide hydrolase cDNA, designated celD, was isolated from a cDNA library of the rumen fungus Neocallimastix patriciarum. The enzyme encoded by celD had endoglucanase, cellobiohydrolase and xylanase activities. Deletion analysis revealed that celD cDNA can be truncated to
Surface adhesion of fusion proteins containing the hydrophobins HFBI and HFBII from Trichoderma reesei
Linder M, et al.
Protein Science, 11(9), 2257-2266 (2002)
K H Jung et al.
Biochemistry and molecular biology international, 44(2), 283-292 (1998-04-08)
A Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was categorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes
A V Gusakov et al.
Biochimica et biophysica acta, 1073(3), 481-485 (1991-04-09)
Using 4-methylumbelliferyl (MUF) beta-D-cellobioside as a substrate, the ability of cellobiohydrolase I from Trichoderma longibrachiatum to catalyze transglycosylation has been demonstrated. At substrate concentrations greater than 2 mM, the formation of MUF-tetrasaccharide was detected using HPLC. In the course of
Methylxanthine Drugs Are Chitinase Inhibitors: Investigation of Inhibition and Binding Modes
Rao FV, et al.
Chemistry & Biology, 12(9), 973-980 (2005)
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