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Merck

I9271

Sigma-Aldrich

5-(Iodoacetamido)fluorescein

≥90% purity (HPLC), powder

Synonym(s):

5-IAF

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About This Item

Empirical Formula (Hill Notation):
C22H14INO6
CAS Number:
Molecular Weight:
515.25
Beilstein:
6543244
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

Pricing and availability is not currently available.

Product Name

5-(Iodoacetamido)fluorescein, ≥90% (HPLC)

Quality Level

Assay

≥90% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to orange

solubility

DMSO: 5 mg/mL

fluorescence

λex 492 nm; λem 515 nm (Mercaptoethanol adduct)
λex 493 nm; λem 522 nm (pH 9.2)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

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Show Differences

1 of 4

This Item
D0531F9551F3651
color

yellow to orange

color

yellow to orange

color

yellow to orange

color

yellow to orange

fluorescence

λex 492 nm; λem 515 nm (Mercaptoethanol adduct), λex 493 nm; λem 522 nm (pH 9.2)

fluorescence

λex 495 nm; λem 516 nm

fluorescence

λex 511-519 nm

fluorescence

-

solubility

DMSO: 5 mg/mL

solubility

methanol: 20 mg/mL, clear to slightly hazy, yellow to orange

solubility

DMSO or DMF: soluble

solubility

acetone: 1 mg/mL, clear, light yellow to yellow

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

powder

form

powder

General description

5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative.

Application

5-(Iodoacetamido)fluorescein is used for the synthesis of fluorescently labeled organelles (nuclei and nuclear matrices), proteins and peptides that include enzymes.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Multisite phosphorylation of the Saccharomyces cerevisiae filamentous growth regulator Tec1 is required for its recognition by the E3 ubiquitin ligase adaptor Cdc4 and its subsequent destruction in vivo.
Bao MZ
Eukaryotic Cell, 9, 31-31 (2010)
Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase.
Tyson PA
The Journal of Biological Chemistry, 264, 726-726 (1989)
Meng M Rowland et al.
Nucleic acids research, 42(14), 9295-9303 (2014-07-14)
The DNA backbone is often considered a track that allows long-range sliding of DNA repair enzymes in their search for rare damage sites in DNA. A proposed exemplar of DNA sliding is human 8-oxoguanine ((o)G) DNA glycosylase 1 (hOGG1), which
Gábor Glatz et al.
The Journal of biological chemistry, 288(12), 8596-8609 (2013-02-06)
Mitogen-activated protein kinase (MAPK) activation depends on a linear binding motif found in all MAPK kinases (MKK). In addition, the PB1 (Phox and Bem1) domain of MKK5 is required for extracellular signal regulated kinase 5 (ERK5) activation. We present the
Alessandro Musenga et al.
Analytical and bioanalytical chemistry, 387(3), 917-924 (2007-01-05)
Glutathione (GSH) is a thiol with an important function in protecting tissue against the oxidative stress which has been related to carcinogenesis in the colon. For this reason the development of probiotic species producing glutathione could be of great interest.

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