I8135
IgM from bovine serum
reagent grade, ≥95% (SDS-PAGE or HPLC), buffered aqueous solution
Synonym(s):
Bovine IgM
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About This Item
Recommended Products
conjugate
unconjugated
Quality Level
grade
reagent grade
Assay
≥95% (SDS-PAGE or HPLC)
form
buffered aqueous solution
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
IgM antibodies are present as pentamers in the serum and are produced in response to antigens
IgM from bovine serum is purified from normal bovine serum by precipitation and gel filtration techniques.
IgM from bovine serum is purified from normal bovine serum by precipitation and gel filtration techniques.
IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It exists as a pentamer and is secreted by peritoneal B cells.
Application
IgM from bovine serum has been used for screening the lipopolysaccharide levels in Limulus amoebocyte lysate (LAL) assay. IgM has been used in flow cytometry assay for detecting FcγRIV expression and immunoglobulin binding.
Purified bovine IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.
Biochem/physiol Actions
IgM plays a key role in engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.
Physical form
Solution in 0.05 M Tris-HCl, 0.15 M sodium chloride and 0.01 M glycine, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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IgE b immune complexes activate macrophages through FcgammaRIV binding
Hirano M, et al.
Nature Immunology, 8(7), 762-762 (2007)
Lipopolysaccharide is a frequent and significant contaminant in microglia-activating factors
Weinstein JR, et al.
Glia, 56(1), 16-26 (2008)
Emerging functions of natural IgM and its Fc receptor FCMR in immune homeostasis
Wang H, et al.
Frontiers in Immunology, 7, 99-99 (2016)
Protective roles of natural IgM antibodies
Gronwall C, et al.
Frontiers in Immunology, 3, 66-66 (2012)
Masayuki Hirano et al.
Nature immunology, 8(7), 762-771 (2007-06-15)
Because functional analysis of Fc receptors (FcRs) relies heavily on mouse models, the identification of another Fcgamma receptor is particularly noteworthy. We demonstrate that FcgammaRIV, identified here as the mouse ortholog of primate FcgammaRIII, required association of the FcR gamma-chain
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