Recommended Products
shelf life
2 yr at 2‑8 °C
Quality Level
pore size
100 nm pore size
capacity
>0.5 mg/column
storage temp.
2-8°C
Related Categories
General description
Kit contains 3 collection tubes for every spin column.
Application
Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.
Features and Benefits
- High Selectivity for higher purity
- Convenient and Ready-to-Use
- Consistent protein binding each use
- Compatible with CelLytic™ cell lysis reagents
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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