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GE17-0404-03

HiTrap® Protein G High Performance

Cytiva 17-0404-03, pack of 2 × 1 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 2 × 1 mL

manufacturer/tradename

Cytiva 17-0404-03

storage condition

(20% Ehtanol)
(20% Ethanol)

parameter

42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

size

avg. part. size

34 μm

cleaning

2-9(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range

3-9(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

capacity

>1 mg binding capacity (lgG/column)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
25 mg binding capacity(human IgG/column)

storage temp.

2-8°C

Related Categories

General description

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.

Features and Benefits

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nimitha R Mathew et al.
Cell reports, 35(12), 109286-109286 (2021-06-24)
B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of

Articles

This page describes automated purification of antibodies using ÄKTA chromatography systems.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

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Protocols

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

This page shows how to prepare samples for purification with affinity chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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