D4522

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

• Synonym(s): Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
• CAS Number: 91080-16-9
• EC Number: 293-507-3
• MDL number: MFCD00151698
• UNSPSC Code: 41106310
• eCl@ss: 32160414
• NACRES: NA.52

Properties

• grade: for molecular biology
• Quality Level: 200
• type: Type XV
• form: lyophilized powder
• storage temp.: −20°C

Description

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Quantity

One mg DNA is approx. 20 A₂₆₀ units.

Preparation Note

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves