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CS0780

Sigma-Aldrich

β-N-Acetylglucosaminidase Assay Kit

sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)

Synonym(s):

Beta-NAG Activity Assay Kit

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About This Item

EC Number:
222-398-7
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 50 reactions (1 mL)
 sufficient for 500 reactions (100 μL)

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... NAGLU(4669)

Application

The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.

Biochem/physiol Actions

N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.

Analysis Note

The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Kit Components Only

Product No.
Description
  • Dilution Buffer 8 mL
  • 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
  • Citrate Buffer Solution, 0.09 M 100 mL
  • p-Nitrophenol Standard Solution, 10 mM 1 mL
  • β-N-Acetylglucosaminidase from Jack beans 1 vial
  • Sodium carbonate 5 g

related product

Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H319,H351,H373

Hazard Classifications

Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral

Target Organs

Liver,Kidney

Storage Class Code

11 - Combustible Solids

Certificates of Analysis (COA)

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Articles

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

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