CLLS1010

SW48 Cells SMAD4 -/-

Human female colon, colorectal adenocarcinoma

• Synonym(s): CLL1008
• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• Product Name: SW48 Cells SMAD4 -/-,
• biological source: human female colon (Source Disease: Colorectal adenocarcinoma)
• Quality Level: 200
• OMIM accession no.: 600993
• storage temp.: −196°C
• Gene Information: human ... SMAD4(4089)

Description

General description

SW48 Cells SMAD4 -/- are colorectal adenocarcinoma, epithelial cells, from a human caucasian female (aged 82 years), with a ZFN knock out modification.

This product corresponds to ATCC Cat. No. CCL-231

Application

Media Renewal changes one to two times per week

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:2 to 1:6

The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.

The cell freezing medium is DMSO 1X (Cat. No. C6164)

Features and Benefits

These SW48 cells are adherent, with a doubling time of approx. 35 hours.

Zinc finger nuclease (ZFN) knock out on chromosome 18q21.1

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Also Available Separately

• SW48 Cells SMAD4 -/- 1 vial
• SW48 Control Cells 1 vial