2-179-E11
from Mouse x Mouse Hybridoma, 92030602, Reactivity Anti-TNF alpha, human )
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About This Item
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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS0
Reactivity
Anti-TNF alpha, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
Cell Line Origin
Mouse NS0 x Mouse BALB/c spleen
Cell Line Description
This strongly neutralising antibody is directed against natural and recombinant human TNF alpha. It does not cross-react with TNF ß. It cross-reacts with simian TNF alpha but not with TNF alpha or ß of other species. It can be paired in a sandwich ELISA with 357-101-4. Disclaimer required.
Immunogen
Highly purified recombinant human tumour necrosis factor alpha
Application
Immunoassays, TNF alpha neutralisation, immunohistochemistry
Culture Medium
RPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS) (heat inactivated)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
Certificates of Analysis (COA)
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