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product name
LL29 (AnHa), 87112508
biological source
human lung
growth mode
Adherent
karyotype
2n = 46; diploid
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian diploid lung
Cell Line Description
Derived from a biopsy taken from a 26 year old Caucasian male with idiopathic pulmonary fibrosis.
Application
Tumourigenicity studies
Culture Medium
Coon′s modified Ham′s F12 or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. A further 9 population doublings expected.
Other Notes
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Certificates of Analysis (COA)
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