C213250

DMT-2′Fluoro-dC(ac) Phosphoramidite

configured for (ÄKTA^® and OligoPilot^®)

• Synonym(s): DMT-2′-F-dC(ac) amidite
• Empirical Formula (Hill Notation): C₄₁H₄₉FN₅O₈P
• CAS Number: 159414-99-0
• Molecular Weight: 789.83
• MDL number: MFCD12912404
• UNSPSC Code: 12352200
• PubChem Substance ID: 329774961
• NACRES: NA.21

Properties

• biological source: non-animal source (no BSE/TSE risk)
• Quality Level: 300
• product line: Proligo Reagents
• Assay: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• form: powder
• technique(s): oligo synthesis: suitable
• impurities: ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤0.5% single Impurity (reversed phase HPLC); ≤3% residual Solvent content
• color: white to off-white
• λ: conforms (UV/VIS Identity)
• suitability: conforms to structure for H-NMR; conforms to structure for LC-MS
• compatibility: configured for (ÄKTA^® and OligoPilot^®)
• nucleoside profile: base: deoxycytidine; base protecting group: acetyl; 2' protecting group: fluoro; 5' protecting group: DMT; deprotection: fast/standard
• storage temp.: −20°C
• SMILES string: COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](F)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=CC(NC(C)=O)=NC3=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C41H49FN5O8P/c1-27(2)47(28(3)4)56(53-25-11-23-43)55-38-35(54-39(37(38)42)46-24-22-36(44-29(5)48)45-40(46)49)26-52-41(30-12-9-8-10-13-30,31-14-18-33(50-6)19-15-31)32-16-20-34(51-7)21-17-32/h8-10,12-22,24,27-28,35,37-39H,11,25-26H2,1-7H3,(H,44,45,48,49)/t35-,37-,38-,39-,56?/m1/s1
• InChI key: CNFKJHKDSRXNFL-UTXREMQHSA-N

Description

General description

DMT-2′Fluoro-dC(ac) Phosphoramidite is a 2′Fluoro phosphoramidite used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.Its key features are-

• Can be employed together with DNA or RNA phosphoramidites
• Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C
• Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
• Consistent lot-to-lot high purity and performance
• Manufactured under a certified ISO 9001 quality system

Legal Information

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable