A9452
Anti-Goat/Sheep IgG−Peroxidase antibody, Mouse monoclonal
clone GT-34, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Goat/Sheep IgG
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
secondary antibodies
clone
GT-34, monoclonal
form
lyophilized powder
species reactivity
sheep, goat, bovine
should not react with
rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human
packaging
vial of 0.5 mL
technique(s)
direct ELISA: 1:30,000
dot blot: 1:160,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
isotype
IgG1
storage temp.
2-8°C
target post-translational modification
unmodified
Looking for similar products? Visit Product Comparison Guide
General description
IgG is the most abundant antibody in human serum.It is found in blood and secreted by the B cells. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
IgGs are glycoprotein antibodies that modulate several immune responses. Goat or sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat/sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components. Moreover, the antibody does not react with the following species: guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components. Moreover, the antibody does not react with the following species: guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.
Application
ELISA using HRP-conjugated monoclonal anti-goat/sheep IgG as the secondary was performed on human T cells.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody has been used for western blot (1:5,000) and ELISA. The product can also be used for dot blot (1:30,000) and IHC (1:100).
Biochem/physiol Actions
IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG enables transplacental transport. It has the longest serum half life. It might be associated with allergy.
Physical form
Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
L Alvarez-Vallina et al.
Human gene therapy, 10(4), 559-563 (1999-03-27)
Engineered T cells expressing chimeric T cell receptors (chTCRs) are of interest for cancer gene therapy but many "cancer antigens" are thought to be unsuitable targets because they are expressed at low levels on normal tissues. We therefore sought to
Li He et al.
The Journal of clinical investigation, 109(2), 213-219 (2002-01-24)
Heparin cofactor II (HCII) is a plasma protein that inhibits thrombin rapidly in the presence of dermatan sulfate, heparan sulfate, or heparin. HCII has been proposed to regulate coagulation or to participate in processes such as inflammation, atherosclerosis, and wound
Shuya Hara et al.
Molecular vision, 16, 2279-2285 (2010-12-09)
To investigate the presence of epithelial sodium channels (ENaC) in rabbit and human conjunctival epithelium and to test the effects of topical amiloride, a potassium-sparing diuretic that blocks the ENaC, on tear quantity in rabbits. Both healthy normal human and
Adrienn Bíró et al.
Immunology, 121(1), 40-50 (2007-01-25)
Several studies have investigated the interactions between C-reactive protein (CRP) and various complement proteins but none of them took into consideration the different structural forms of CRP. The aim of our study was to investigate whether the different antigenic forms
Eeva Kaisa Rajakylä et al.
Nature communications, 6, 5978-5978 (2015-01-15)
Controlled transport of macromolecules between the cytoplasm and nucleus is essential for homeostatic regulation of cellular functions. For instance, gene expression entails coordinated nuclear import of transcriptional regulators to activate transcription and nuclear export of the resulting messenger RNAs for
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service