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93834

Sigma-Aldrich

2-Chloro-4-nitrophenyl-α-D-maltotrioside

chromogenic, ≥95% (HPLC), powder or crystals

Synonym(s):

2-chloro-4-nitrophenyl O-α-D-glucopyranosyl-(1→4)-O-α-D-glucopyranosyl-(1→4)-α-D-glucopyranoside, CNP-G3

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About This Item

Empirical Formula (Hill Notation):
C24H34ClNO18
CAS Number:
118291-90-0
Molecular Weight:
659.98
MDL number:
MFCD11045274
UNSPSC Code:
12352204
PubChem Substance ID:
329770276
NACRES:
NA.32

product name

2-Chloro-4-nitrophenyl-α-D-maltotrioside, ≥95% (HPLC)

Quality Level

200

Assay

≥95% (HPLC)

form

powder or crystals

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O[C@@H]2CO)O[C@H]3[C@H](O)[C@@H](O)[C@H](O[C@@H]3CO)Oc4ccc(cc4Cl)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C24H34ClNO18/c25-8-3-7(26(37)38)1-2-9(8)39-22-18(35)15(32)20(11(5-28)41-22)44-24-19(36)16(33)21(12(6-29)42-24)43-23-17(34)14(31)13(30)10(4-27)40-23/h1-3,10-24,27-36H,4-6H2/t10-,11-,12-,13-,14+,15-,16-,17-,18-,19-,20-,21-,22+,23-,24-/m1/s1

InChI key

KMYYNUOXSFGLNX-XFNLHOCBSA-N

Application

2-Chloro-4-nitrophenyl-α-D-maltotrioside has been used as a substrate in assays to determine the enzymatic activity of α-amylase.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sergio Alcalá-Alcalá et al.
International journal of pharmaceutics, 489(1-2), 139-147 (2015-05-06)
This work presents an evaluation of the adsorption/infiltration process in relation to the loading of a model protein, α-amylase, into an assembled biodegradable polymeric system, free of organic solvents and made up of poly(D,L-lactide-co-glycolide) acid (PLGA). Systems were assembled in
T Suganuma et al.
Carbohydrate research, 303(2), 219-227 (1997-11-14)
The degradation mechanism of a synthetic substrate, 2-chloro-4-nitrophenyl alpha-maltotrioside (CNP-G3), by human salivary alpha-amylase (HSA) was investigated by kinetic and product analyses. It was observed that the enzyme attacked the various CNP-maltooligosaccharides (CNP-G3 to CNP-G6) releasing free CNP. Addition of
Reference values for alpha-amylase in human serum and urine using 2-chloro-4-nitrophenyl-alpha-D-maltotrioside as substrate.
D Balsells et al.
Clinica chimica acta; international journal of clinical chemistry, 274(2), 213-217 (1998-08-07)
Klaus Lorentz
Clinical chemistry and laboratory medicine, 40(8), 781-785 (2002-10-24)
The advanced knowledge on substrate cleavage by human alpha-amylases promotes the development of chromogenic maltotriosides exclusively cleaved at the aglycone bond. Three essentials are required for this type of binding at the active site of the enzyme: (i) A minimal
K Lorentz et al.
Clinical chemistry and laboratory medicine, 37(11-12), 1053-1062 (2000-03-22)
We present the adaptation of an IFCC method for alpha-amylase using 2-chloro-4-nitro-phenyl-alpha-D-maltotrio-side as substrate (1) suited for routine work at 37 degrees C. In the assay, a constant proportion of substrate, i. e. 92%, is directly converted to 2-chloro-4-nitrophenol and
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