88417

MTT Formazan

MTT reduction end product, powder

• Synonym(s): 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan
• Empirical Formula (Hill Notation): C₁₈H₁₇N₅S
• CAS Number: 57360-69-7
• Molecular Weight: 335.43
• MDL number: MFCD00080731
• UNSPSC Code: 12352204
• PubChem Substance ID: 329769650
• NACRES: NA.83

Properties

• product name: MTT Formazan, MTT reduction end product
• form: powder
• solubility: chloroform: 10 mg/mL, clear, dark violet
• SMILES string: Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
• InChI: 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
• InChI key: GIFKVQOPAJDJLO-SZVLODPRSA-N

Description

Application

MTT Formazan has been used in cell viability assay.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Other Notes

Reviews: Tetrazolium salts and formazans

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves