biological source
mouse (Engelbreth-Holm-Swarm (EHS) mouse tumor)
sterility
sterile
Assay
≥90% (SDS-PAGE)
concentration
6 mg/mL
technique(s)
cell culture | mammalian: suitable
solubility
water: miscible
shipped in
dry ice
storage temp.
−70°C
General description
3-D Culture Matrix™ Mouse Laminin I may be used as a gel on which to grow cells, or as a media additive alone or in concert with other basement membrane components, to study cellular growth and differentiation in three dimensions in vitro. To offer the most standardized Laminin I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of 6 mg/ml (by absorbance and extinction coefficient).
Physical form
Solution in Dulbecco′s Modified Eagle′s medium containing 10 μg/ml gentamycin sulfate and no phenol red.
Other Notes
Functional Assay:
3-D Culture: Laminin I promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
Cell Attachment: Tested for the ability to promote cell attachment and spreading of MG63 human osteosarcoma cells.
3-D Culture: Laminin I promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
Cell Attachment: Tested for the ability to promote cell attachment and spreading of MG63 human osteosarcoma cells.
Sterility Testing:
No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test.No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test.No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
Storage Conditions and Product Stability:
Product is stable for a minimum of 3 months from date of shipment when stored at -80 °C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles.
Product is stable for a minimum of 3 months from date of shipment when stored at -80 °C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles.
Legal Information
3-D Culture Matrix is a trademark of Trevigen, Inc.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Daniel Rüdiger et al.
Cell reports, 32(6), 108015-108015 (2020-08-14)
Endothelial tube formation on a reconstituted basement membrane (Matrigel) is a well-established in vitro model for studying the processes of angiogenesis and vasculogenesis. However, to date, the organizing principles that underlie the morphogenesis of this network and that shape the initial
Ruilin Tian et al.
Neuron, 104(2), 239-255 (2019-08-20)
CRISPR/Cas9-based functional genomics have transformed our ability to elucidate mammalian cell biology. However, most previous CRISPR-based screens were conducted in cancer cell lines rather than healthy, differentiated cells. Here, we describe a CRISPR interference (CRISPRi)-based platform for genetic screens in
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service