50484

Methylene Blue solution

suitable for microbiology

• Synonym(s): Ehrlich’s reagent III, Loeffler’s Methylene Blue
• Empirical Formula (Hill Notation): C₁₆H₁₈ClN₃S
• CAS Number: 61-73-4
• Molecular Weight: 319.85
• Beilstein: 3641570
• MDL number: MFCD00012111
• UNSPSC Code: 12171500
• PubChem Substance ID: 329757603
• NACRES: NA.47

Properties

• shelf life: limited shelf life, expiry date on the label
• concentration: 0.5% in H₂O (0.5 g/100 mL)
• technique(s): microbe id | staining: suitable
• application(s): microbiology
• suitability: Bacillus spp.; Corynebacterium spp.; bacteria
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChI key: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Description

General description

Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).

Components

reagent contains:
methylene blue 0.5 g
distilled water 100 ml

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves