05121
Heart Infusion Broth
NutriSelect® Plus, suitable for microbiology
Synonym(s):
HIB
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About This Item
UNSPSC Code:
41171607
NACRES:
NA.85
Recommended Products
sterility
non-sterile
Quality Level
form
powder
shelf life
limited shelf life, expiry date on the label
composition
beef heart (infusion from 500 g), 10 g/L
sodium chloride, 5.0 g/L
tryptose, 10.0 g/L
packaging
bottle of 500 g
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
color
light yellow
final pH
7.4±0.2 (25 °C)
General description
Heart Infusion Broth, containing infusion from the beef heart, is used for the isolation and cultivation of a wide variety of fastidious organisms.The fastidious organisms having challenging nutritional requirements could be cultivated on infusion media. A liquid medium containing an infusion of meat was one of the first media used for the cultivation of bacteria. The heart infusion media requires no further supplements to cultivate fastidious bacteria. The media contains Tryptose and beef heart infusions, which provide nitrogenous compounds, amino acids, and other essential growth nutrients required for the growth pathogenic bacteria. Sodium chloride maintains the osmotic equilibrium of the medium. Heart Infusion Broth is also recommended for Vibrio cholerae and Vibrio species and can be used as the base in carbohydrate fermentation tests. This medium is also used for the isolation and enumeration of hemolytic Streptococci in milk.
Application
Heart Infusion Broth is used for general laboratory purposes for the cultivation of fastidious organisms from various test samples.
Features and Benefits
NutriSelect® Plus are powdered culture media offering
- Quality control with growth promotion test according to industry standards
- Quality assurance acc. to ISO 9001
- Cost-efficient powdered media
Preparation Note
Suspend 25 g in 1000 ml of purified water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. If desired 5% sterile defibrinated blood may be added. Mix well and pour sterile petri plates or tubes.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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