MABT163

Anti-γ-Tubulin Antibody

mouse monoclonal, 6H3.1

• Synonym(s): Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• product name: Anti-Gamma-1-Tubulin, clone 6H3.1 Antibody, clone 6H3.1, from mouse
• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6H3.1, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001061
• UniProt accession no.: P23258
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TUBG1(7283)

Description

General description

Gamma-1-Tubulin, also known as Gamma-tubulin complex component 1, GCP-1 or Tubulin gamma-1 chain, and encoded by the gene TUBG1/TUBG, is a form of tubulin protein and a component of microtubules. Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome and plays a major role in the organization of the microtubule cytoskeleton. Gamma-1-Tubulin is part of the pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, and it is also involved in centrosome duplication and spindle formation. Interestingly, gamma tubulin is often up regulated but improperly compartmentalized in many cancer cells including non-small cell lung cancer. Mutations in Gamma-1-Tubulin gene are associated with developmental cortical defects including microcephaly and malformations of cortical development (MCD). Gamma-1-Tubulin is localized to the cytoplasm and cytoskeleton components and expressed in a variety of cell types and tissues.

Immunogen

GST-tagged recombinant protein corresponding to human Gamma-1-Tubulin.

Application

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Gamma-1-Tubulin in NIH/3T3 cells.

Alexa Fluor^™ is a registered trademark of Life Technologies.

This Anti-Gamma-1-Tubulin antibody, clone 6H3.1 is validated for use in western blotting & ICC for the detection of Gamma-1-Tubulin.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Gamma-1-Tubulin in 10 µg of HeLa cell lysate.

Target description

~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable