MABS2226

Anti-ELOVL3 Antibody, clone V61 P2B3 D10

• Synonym(s): Elongation of very long chain fatty acids protein 3;EC:2.3.1.199;3-keto acyl-CoA synthase ELOVL3;Cold-inducible glycoprotein of 30 kDa;ELOVL fatty acid elongase 3;ELOVL FA elongase 3;Very long chain 3-ketoacyl-CoA synthase 3;Very long chain 3-oxoacyl-CoA
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: V61 P2B3 D10, monoclonal
• mol wt: calculated mol wt 31.5 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100 μL
• technique(s): ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal
• Protein ID accession no.: NP_689523
• UniProt accession no.: Q9HB03
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... ELOVL3(83401)

Description

General description

Elongation of very long chain fatty acids protein 3 (UniProt: Q9HB03; also known as EC:2.3.1.199, 3-keto acyl-CoA synthase ELOVL3, Cold-inducible glycoprotein of 30 kDa, ELOVL fatty acid elongase 3, ELOVL FA elongase 3, Very long chain 3-ketoacyl-CoA synthase 3, Very long chain 3-oxoacyl-CoA synthase 3) is encoded by the ELOVL3 (also known as CIG30) gene (Gene ID: 83401) in human. ELOVL3 is a multi-pass membrane protein of endoplasmic reticulum (ER). It catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. Its C-terminal di-lysine motif (aa 266-270) confers endoplasmic reticulum localization. This ER-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. It exhibits higher activity towards C18:0 acyl-CoAs. It is strongly expressed in normal skin, breast, colon, liver tissues. A decrease in the expression of ELOVL3 is reported in colorectal tumors compared to normal colorectal epithelium. ELOVL3 is reported to be involved in the regulation of the progression of adipogenesis through activation of peroxisome proliferator-activated receptor (PPAR) in mouse adipocytic 3T3-L1 cells. Its levels are elevated more than200-fold in cold-stressed animals. (Ref.: Alnabulsi, A., et al. (2019). Int. J. Cancer. 145(4);1138-1147; Kobayashi, T., and Fujimori, K. (2012). 302(12); E1461-E1471; Westerberg, R., et al. (2006). J. Biol. Chem. 281(8); 4958-4968).

Specificity

Clone V61 P2B3 D10 is a mouse monoclonal antibody that detects Elongation of very long chain fatty acids protein 3 (ELOVL3). It targets and epitope within 9 amino acids from the N-terminal region.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to 9 amino acids from the N-terminal region of human Elongation of very long chain fatty acids protein 3 (ELOVL3).

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human colon cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected ELOVL3 in human colon cancer tissue sections.

Tested Applications

Western Blotting Analysis: A representative lot detected ELOVL3 in Western Blotting applications (Alnabulsi, A., et al. (2019). Int J Cancer. 45(4):1138-1147).

ELISA Analysis: A representative lot detected ELOVL3 in ELISA applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Immunohistochemistry Applications: A representative lot detected ELOVL3 in Immunohistochemistry applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ELOVL3, clone V61 P2B3 D10, Cat. No. MABS2226, is a mouse monoclonal antibody that detects ELOVL3 and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable