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MABN68

Sigma-Aldrich

Anti-PSD95 Antibody, clone K28/43

clone K28/43, from mouse

Synonym(s):

Postsynaptic density protein 95, Synapse-associated protein 90, Tax interaction protein 15, discs large homolog 4, discs, large homolog 4 (Drosophila), post-synaptic density protein 95

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

K28/43, monoclonal

species reactivity

rat

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), human (immunogen homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DLG4(1742)

General description

PSD95 (postsynaptic density 95) represent a class of anchoring proteins which serve to localize various neuronal ion channels to post-synaptic densities. PSD95 and related proteins contain PDZ domains, which interact with NMDA receptors and voltage-gated K+ channels via a ET/SXV motif in the cytoplasmic carboxyl terminus of the receptor channels.

Immunogen

Recombinant protein corresponding to human PSD95.

Application

Detect PSD95 using this Anti-PSD95 Antibody, clone K28/43 validated for use in IH, WB & IC.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected PSD95 in rat cortex tissue.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Maeda, T., et al. (2005). Invest. Ophthalmol. Vis. Sci. 46(11):4320-4327.)
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Quality

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PSD95 on 10 µg of rat brain tissue lysate.

Target description

~95 kDa observed.

Linkage

Replaces: 04-1066

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lu Yang et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 24(48), 10846-10857 (2004-12-03)
The specification and organization of glutamatergic synaptic transmission require the coordinated interaction among glutamate receptors and their synaptic adaptor proteins closely assembled in the postsynaptic density (PSD). Here we investigated the interaction between NMDA receptors and metabotropic glutamate receptor 5
Vibeke M Bruinenberg et al.
Nutrients, 8(4), 185-185 (2016-04-23)
The inherited metabolic disease phenylketonuria (PKU) is characterized by increased concentrations of phenylalanine in the blood and brain, and as a consequence neurotransmitter metabolism, white matter, and synapse functioning are affected. A specific nutrient combination (SNC) has been shown to
Optimized protocol for retinal wholemount preparation for imaging and immunohistochemistry.
Ivanova, E; Toychiev, AH; Yee, CW; Sagdullaev, BT
Journal of Visualized Experiments null
Min-Jian Wang et al.
Medical science monitor : international medical journal of experimental and clinical research, 25, 4627-4638 (2019-07-04)
<strong>BACKGROUND</strong> Subclinical epileptiform discharges (SEDs) are defined as epileptiform electroencephalographic (EEG) discharges without clinical signs of seizure in patients. The subthreshold convulsant discharge (SCD) is a frequently used model for SEDs. This study aimed to investigate the effect of levetiracetam
Pranesh Padmanabhan et al.
eLife, 8 (2019-06-27)
The Src kinase Fyn plays critical roles in memory formation and Alzheimer's disease. Its targeting to neuronal dendrites is regulated by Tau via an unknown mechanism. As nanoclustering is essential for efficient signaling, we used single-molecule tracking to characterize the

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