MABN674
Anti-PSIP1 Antibody, clone 6E4
ascites fluid, clone 6E4, from mouse
Synonym(s):
PSIP1 protein
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
6E4, monoclonal
species reactivity
human, rat, monkey
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PSIP1(11168)
General description
PSIP1, also known as coactivator p75, as well as numerous other synonyms named after their original discoveries including, PC4 and SFRS1 interacting protein 1, lens epithelium-derived growth factor (LEDGF/p75), dense fine speckles 70kD protein (DFS 70), and transcriptional coactivator p75/p52, is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It is particularly important in lens epithelial cell gene regulation and stress responses, and even though initially thought to be limited to the lens of the eye, PSIP1 is now known to be widely expressed in most tissues and has no direct role in the development of lens epithelium. It also gained research interest as the focal point for HIV integration and appears to be one of the few integrase interactor proteins whose knockdown limits HIV integration and propagation. Moreover, PSIP1 is an anti-apoptotic protein and appears to play a protective role during stress-induced apoptosis. There are three isoforms of the PSIP1 in humans, PSIP1, PSIP2 or p52, and a third isoform which is unnamed. The PSIP1 isoforms seem to vary in their precise functions somewhat with isoform 2 being the most commonly expressed and most abundant. Expression is localized to the nucleus. EMD-Millipore’s anti-PSIP1mouse monoclonal, clone 6.00E+04 has been tested on human, paraffin embedded immunohistochemical sections, immunocytochemical immunofluorescence studies using mouse NIH3T3 cells, western blot using human, mouse, and rat cells line lysates, and ELISA using purified antigen.
Immunogen
Purified recombinant fragment of human PSIP1 expressed in E. Coli.
Application
Detect PSIP1 using this mouse monoclonal antibody, Anti-PSIP1 Antibody, clone 6E4 validated for use in western blotting, IHC & Immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected PSIP1 in NIH/3T3 cells.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PSIP1 in rat cerebellum, rat hippocampus, and human cerebellum tissues.
Optimal working dilutions must be determined by end user.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PSIP1 in rat cerebellum, rat hippocampus, and human cerebellum tissues.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Quality
Evaluated by Western Blotting in HepG2, Jurkat, K562, Cos7, PC-12, HeLa, and NIH/3T3 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PSIP1 in HepG2, Jurkat, K562, Cos7, PC-12, HeLa, and NIH/3T3 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PSIP1 in HepG2, Jurkat, K562, Cos7, PC-12, HeLa, and NIH/3T3 cell lysates.
Target description
~75 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physical form
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HepG2, Jurkat, K562, Cos7, PC-12, HeLa, and NIH/3T3 cell lysates
HepG2, Jurkat, K562, Cos7, PC-12, HeLa, and NIH/3T3 cell lysates
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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