MABF2166

Anti-Chlamydial MOMP Antibody, clone L21-10

clone L21-10, from mouse

• Synonym(s): Chlamydia major membrane protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: L21-10, monoclonal
• species reactivity: Chlamydia, bacteria
• packaging: antibody small pack of 25 μg
• technique(s): dot blot: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aλ
• target post-translational modification: unmodified

Description

General description

Chlamydia trachomatis is a Gram-negative bacterium that is responsible for sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV). Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants. These are identified based on serological reactivity of the epitopes on their outer membrane. Intracellularly chlamydia replicates within a vacuole. Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles. This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell. Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate. Clone L21-10 specifically detects major outer membrane protein (MOMP) from Chlamydia genus. It displays strong reactivity with Chlamydial serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2. MOMP, a cysteine-rich molecule, plays a vital role in maintaining the structural and functional properties of the chlamydial outer membrane. It contains determinants that confer type, subspecies, and species antigenic properties to the protein. (Ref.: Zhang, YX et al., (1987). J. Immunol. 138(2); 575-581).

Specificity

Clone L21-10 specifically detects outer membrane protein from Chlamydia genus. It displays strong reactivity with serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2.

Immunogen

Formalin killed Chlamydia trachomatis (L2/LGV-434).

Application

Anti-Chlamydial MOMP, clone L21-10, Cat. No. MABF2166, is a mouse monoclonal antibody that detects Chlamydial major outer membrane protein (MOMP) and has been tested for use in Dot Blot, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: A representative lot immunoprecipiated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).

Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Weber, M.M., et. al. (2017). Cell Rep. 19(7):1406-1417; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).

Western Blotting Analysis: A representative lot detected Chlamydial MOMP in Western Blotting applications (Baehr, W., et. al. (1988). Proc Natl Acad Sci USA. 85(11):4000-4; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).

Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).

Research Category
Inflammation & Immunology

Quality

Evaluated by Western Blotting in lysates from HeLa cells infected with Chlamydia trachomatis serovar L2 (LGV 434 L2) .

Western Blotting Analysis: 2 µg/mL of this antibody detected chlamydial Chlamydial MOMP in lysates from HeLa cells infected with Chlamydia trachomatis serovar L2 (LGV 434 L2) .

Target description

~43 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.