MABE1919
Anti-TNRC6ABC Antibody, clone 7A9
clone 7A9, from rat
Synonym(s):
Trinucleotide repeat-containing gene 6A, 6B, and 6C, CAG repeat protein 26, EMSY interactor protein, GW182 autoantigen, Protein GW1, Glycine-tryptophan protein of 182 kDa
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7A9, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μg
technique(s)
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
target post-translational modification
unmodified
Gene Information
human ... TNRC6B(23112)
General description
Trinucleotide repeat-containing gene 6A, 6B, and 6C proteins (UniProt: Q8NDV7; Q9UPQ9; Q9HCJ0) are encoded by the TNRC6A, TNRC6B, and TNRC6C genes (Gene ID: 27327, 23112, and 57690), respectively in human. TNRC6 proteins are ubiquitously distributed and play a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). TNRC6 proteins are required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. They also serve as a scaffolding proteins and associate with argonaute proteins bound to partially complementary mRNAs. They can simultaneously recruit CCR4-NOT and PAN deadenylase complexes. TNRC6 proteins are predominantly found in the cytoplasm under steady state conditions, however, they are also found in the nucleus and their nuclear import is mediated via the importin pathways. TNRC6A is shown to posses both nuclear localization (NLS) as well a nuclear export signal (NES). In case of mutated NES, TNRC6A is shown to be retained in the nucleus. TNRC6 proteins contain RRM domains (aa 1781-1853; 1648-1720; and 1563-1632 for TNRC6A, 6B, and 6C, respectively). Clone 7A9 recognizes all three TNRC6 proteins. (Ref.: Schraivogel, D., et al. (2015). Nucleic Acids Res. 43(15); 7447-7461).
Specificity
Clone 7A9 is a rat monoclonal antibody that detects human Trinucleotide repeat-containing gene 6A, 6B, and 6C proteins. It target epitopes with in RRM domains.
Immunogen
Recombinant RRM domain regions from human TNRC6A, TNRC6B, and TNRC6C.
Application
Anti-TNRC6ABC, clone 7A9, Cat. No. MABE1919, is a highly specific rat monoclonal antibody that targets TNRC6 and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Immunofluorescence Analysis: A representative lot detected TNRC6ABC in Immunofluorescence applications (Schraivogel, D., et. al. (2015). Nucleic Acids Res. 43(15):7447-61).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TNRC6ABC in Immunoprecipitation applications (Quevillon Huberdeau, M., et. al. (2017). EMBO J. 36(14):2088-2106).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TNRC6ABC in Immunoprecipitation applications (Quevillon Huberdeau, M., et. al. (2017). EMBO J. 36(14):2088-2106).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in Induced Stably transfected HEK293 cell lysates.
Western Blotting Analysis: 4 µg/mL of this antibody detected TNRC6 in lysate from stably induced HEK 293 cells transfected with TNRC6A, but not in uninduced HEK 293 cells.
Western Blotting Analysis: 4 µg/mL of this antibody detected TNRC6 in lysate from stably induced HEK 293 cells transfected with TNRC6A, but not in uninduced HEK 293 cells.
Target description
~175-210 kDa observed; 210.30,194.00, 175.96 kDa for TNRC6A, 6B, and 6C, respectively. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Daniel Schraivogel et al.
Nucleic acids research, 43(15), 7447-7461 (2015-07-15)
MicroRNAs (miRNAs) guide Argonaute (Ago) proteins to distinct target mRNAs leading to translational repression and mRNA decay. Ago proteins interact with a member of the GW protein family, referred to as TNRC6A-C in mammals, which coordinate downstream gene-silencing processes. The
Miguel Quévillon Huberdeau et al.
The EMBO journal, 36(14), 2088-2106 (2017-06-25)
Argonaute proteins associate with microRNAs and are key components of gene silencing pathways. With such a pivotal role, these proteins represent ideal targets for regulatory post-translational modifications. Using quantitative mass spectrometry, we find that a C-terminal serine/threonine cluster is phosphorylated
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