MAB4385
Anti-TRA-1-85 Antibody, blood group Antigen Ok(a), clone TRA-1-85
clone TRA-1-85, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TRA-1-85, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
input
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
Related Categories
General description
The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells.
Specificity
This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed.
SPECIES REACTIVITIES:
Refer to comments regarding specificity
SPECIES REACTIVITIES:
Refer to comments regarding specificity
Immunogen
2102Ep human Embryonal Carcinoma Cells
Epitope: blood group antigen Ok(a)
Application
Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB.
FACS Analysis: a starting range of 5-20 μg/ml is suggested.
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by the end user.
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
Target description
35-69 kDa
Physical form
Format: Purified
Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide
Storage and Stability
Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months
Analysis Note
Control
Hemopoietic cells and a wide range of normal human tissues
Hemopoietic cells and a wide range of normal human tissues
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Biochemical and genetic analysis of the OKa blood group antigen.
Williams, B P, et al.
Immunogenetics, 27, 322-329 (1988)
Surface antigens of human embryonic stem cells: changes upon differentiation in culture
Draper J.S. et al.
Journal of Anatomy, 200 (Pt 3), 249-258 (2002)
Jee Myung Yang et al.
Experimental & molecular medicine, 53(4), 631-642 (2021-04-09)
Retinal degenerative disorders, including age-related macular degeneration and retinitis pigmentosa (RP), are characterized by the irreversible loss of photoreceptor cells and retinal pigment epithelial (RPE) cells; however, the long-term effect of implanting both human induced pluripotent stem cell (hiPSC)-derived RPE
Budd A Tucker et al.
eLife, 2, e00824-e00824 (2013-08-31)
Next-generation and Sanger sequencing were combined to identify disease-causing USH2A mutations in an adult patient with autosomal recessive RP. Induced pluripotent stem cells (iPSCs), generated from the patient's keratinocytes, were differentiated into multi-layer eyecup-like structures with features of human retinal
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