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Key Documents

MAB1912

Sigma-Aldrich

Anti-Procollagen Type I Antibody, NT, clone M-58

culture supernatant, clone M-58, Chemicon®

Synonym(s):

Anti-Procollagen Antibody, Clone M-58 Anti-Procollagen, Type I Procollagen Detection

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

culture supernatant

clone

M-58, monoclonal

species reactivity

human

should not react with

rat, hamster, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG2α

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... COL1A1(1277)

Specificity

Recognizes Human pro-collagen I. MAB1912 labels the amino-terminal pro-peptide.

Application

Detect Procollagen Type I using this Anti-Procollagen Type I Antibody, N-terminus, clone M-58 validated for use in IH(P).
Immunohistochemistry: 1:10,000-1:50,000 (fresh frozen tissue sections.)

Suitable for formaldehyde/formalin fixed paraffin sections, suggested starting dilution 1:100-1:1000, after treatment with 1% trypsin, 20 minutes at room temperature. Does not stain mature collagen fibers in tissue. Localized intracellularly in cells producing pro-collagen I. Not recommended for Western Blots Optimal working dilutions must be determined by end user.

Analysis Note

Control
Skin tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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