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Key Documents

MAB1340

Sigma-Aldrich

Anti-Collagen Type I Antibody, clone C11

ascites fluid, clone C11, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

C11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable

isotype

IgG

NCBI accession no.

NM_000088.3

UniProt accession no.

P02452

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL1A1(1277)

Specificity

Recognizes both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and II in ELISA, RIA or immunoblotting.

Application

Detect Collagen Type I using this Anti-Collagen Type I Antibody, clone C11 validated for use in ELISA, IH.
Immunohistochemical staining of 10 μm thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is not recommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.

ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Antibody suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.

Optimal working dilutions must be determined by the end user.

Physical form

Immunoglobulin Purified from ascites by ammonium sulfate precipitation and chromatography on DEAE-cellulose. . Lyophilized, reconstitute with 1 mL of distilled water.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360FD

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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